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Hakim Health Sys Res 2007, 10(1): 61-65 Back to browse issues page
Production of plasmid-mediated ß-lactamases in Pseudomonas aeruginosa isolated from burn patients
Abstract:   (34722 Views)

Introduction: Pseudomonas aeruginosa is the most important pathogen causing nosocomial infections in burn patients. The existence of ß-lactamase producing isolates exhibiting resistance to most ß-lactam antimicrobial agents greatly complicates the clinical management of patients infected with such isolates. The aim of this study was to evaluate plasmid mediated ß-lactamase production in Pseudomonas aeruginosa isolates.

Methods: 80 isolatedPseudomonas aeruginosa were isolated from patients with nosocomial infections admitted to Motahari Burn Center in Tehran from March 2004 to February 2005. ß-lactamase production was determined by Iodomertric test, and plasmids were extracted by alkaline lyses method. Electroporation was used for transfection of E. coli DH5α with extracted plasmids.

Results: ß-lactamase production was positive in all Pseudomonas aeruginosa isolates. Most of the strains (97.5%) showed between one and three plasmid bands in agarose gel. Only large plasmids were transferred to E. coli DH5α by electroporation, and ß-lactamase production was positive in all of transformed E. coli DH5α.

Conclusion: The results indicate that the ß-lactamase producing Pseudomonas aeruginosa isolates are predominant in the Motahari Burn Center, and the plasmids commonly involve in ß-lactamase production. Therefore, these plasmids may transfer resistance to the same or different species.

 Hakim Research Journal 2007 10(1): 61- 65.

Keywords: Pseudomonas aeruginosa, beta-lactamase, Iodometric test, plasmid.
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Type of Study: Original | Subject: General
Received: 2008/01/2
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Production of plasmid-mediated ß-lactamases in Pseudomonas aeruginosa isolated from burn patients. Hakim Health Sys Res . 2007; 10 (1) :61-65
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Volume 10, Issue 1 (4-2007) Back to browse issues page
فصلنامه پژوهش حکیم Hakim Research Journal
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