[Home ] [Archive]    
:: Volume 9, Issue 1 (4-2006) ::
Hakim Health Sys Res 2006, 9(1): 38-44 Back to browse issues page
Immunological evaluation of OMP-F vesicle of Pseudomonas aeruginosa CSBPI: 16-190 as a protective vaccine in mice model
Abstract:   (31455 Views)

Introduction: Prevention and control of Pseudomonas aeruginosa infections are still among serious nosocomial problems worldwide, since chemotherapeutic control of the infection generally fails. Therefore, using a safe and reliable vaccine against all wild Pseudomonas aeruginosa serotypes is the only solution to overcome this problem.

Methods: 300 Pseudomonas aeruginosa strains were isolated from patients admitted to four Tehran hospitals. Using standard O-specific typing sera, they were all grouped in 16 out of 17 known Pseudomonas aeruginosa serotypes. The 16 serotypes were lyophilized and each given a code according to the Collection of Standard Bacteria of Pasteur Institute of Iran (CSBPI) for further investigation. Among all clinical samples, CSBPI: 16-190 was the most prevalent Pseudomonas aeruginosa serotype which showed a high agglutination titer against homologous O-specific typing sera. This serotype was selected for extraction of Pseudomonas aeruginosa major outer membrane vesicles (OMP-F).OMP-F vesicles were extracted and purified by deoxycholate ultracentrifuge- differentiation technique. After molecular evaluation, protective activities and safety of the OMP-F vesicles were determined in animal models.

Results: Preset investigation reveals that, purified OMP-F vesicles of Pseudomonas aeruginosa CSBPI: 16-190 is able to induce a high level of protection against all 16 live homologous and heterologous native Iranian Pseudomonas aeruginosa serotypes. Besides, it was pyrogen free and did not produce any detectable abnormal toxicity in rabbits, mice and guinea pigs.

Discussion: The results showed that purified OMP-F vesicles of Pseudomonas aeruginosa CSBPI: 16-190 can be considered a safe and protective immunogen in vaccine therapy against infections caused by all native Pseudomonas aeruginosa immunotypes in Iran.

 Hakim Research Journal 20069(1):38-44.

Keywords: OMP-F vesicles, Pseudomonas aeruginosa, vaccine.
Full-Text [PDF 306 kb]   (4161 Downloads)    
Type of Study: Original |
Received: 2008/01/9
Add your comments about this article
Your username or Email:

CAPTCHA code


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Immunological evaluation of OMP-F vesicle of Pseudomonas aeruginosa CSBPI: 16-190 as a protective vaccine in mice model. Hakim Health Sys Res . 2006; 9 (1) :38-44
URL: http://hakim.hbi.ir/article-1-343-en.html


Volume 9, Issue 1 (4-2006) Back to browse issues page
فصلنامه پژوهش حکیم Hakim Research Journal
Persian site map - English site map - Created in 0.1 seconds with 30 queries by YEKTAWEB 3862